Glutathione-S-transferase purification

Glutathione-S-transferase purification (GST) is a technique used in genetic engineering to purify specific proteins from the genetically engineered organism used to express them. A recombinant protein is fused to a member of the gluathione-S-transferase family that binds glutathione then purified with affinity chromatography on a glutathione bound immobile phase. After the fusion protein is eluted with excess glutathione, the GST fragment can be cleaved off or left in place if it does not interfere with the protein's function.